With the release of Sigma's proteomics grade Trypsin Spin Column, waiting on trypsin to digest samples is no longer the bottleneck prior to mass spectrometry analysis
The Trypsin Spin Column reduces the time to digest complex protein mixtures from as much as 18 hours with conventional methods to only 15 minutes.
Potential discoveries can now be made in minutes rather than hours or days.
"With this new trypsin format, mass spectrometrists will now have the flexibility to analyze important samples within minutes," said Jennifer Williams, product manager for quantitative proteomics.
"It is the easiest and fastest method for protein sample preparation currently available on the market.
"The Trypsin Spin Column exemplifies our commitment to providing cutting-edge proteomics tools to the research community and to the development of innovative products in order to progress scientific research".
The Trypsin Spin Column, proteomics grade, contains highly purified, TPCK-treated porcine trypsin, immobilised on a chemically modified silica support to minimize non-specific absorption.
This enables protein trypsination in one simple step; samples are ready for immediate analysis by mass spectrometry.
10-100 micrograms of protein sample are applied to the Spin Column, where they are digested with great efficiency and specificity.
15 minutes later, peptide fragments are eluted, ready for MS analysis and requiring no additional clean-up.
Quality of MS data obtained using the Trypsin Spin Column is equal to, and in some cases better than, that obtained with a traditional digest.
The launch of the Trypsin Spin column is an innovative addition to Sigma-Aldrich's extensive product portfolio providing its customers with an important tool for the acceleration of results in fast-moving fields such as drug discovery and proteomics.
