Chromatrap has published an application note demonstrating how its solid state patented technology enables ChIP assays using as few as 1000 cells per immunoprecipitation.
Large cell numbers are often required for performing ChIP, which can be challenging when testing limited starting material. Some of the existing techniques for tackling small amounts of sample, rare cell types or difficult cell types which provide
low yield of chromatin are long and complex, and involve DNA amplification or the spiking of samples with chromatin from another source.
In the application note, the authors demonstrate how the sensitivity and dynamic range of the Chromatrap ChIP-seq kit allows users to perform ChIP from as little as 1000 cells with no difference in the level of enrichment, independent of starting cell number.
