Beckman Coulter Life Sciences has expanded the multi-parameter capabilities of its patented CytoFLEX flow cytometer technology with the CytoFLEX LX.
With up to six lasers and 21 fluorescent channels, the CytoFLEXLX is designed to deliver even more parameters with the same exceptional fluorescence sensitivity and superior nanoparticle detection demanded by high end researchers.
Beckman Coulter's CytoFLEX flow cytometer was introduced two years ago and now offers 47 configurations through the CytoFLEX, CytoFLEX S, and now the CytoFLEX LX systems.
With the CytoFLEX LX series, the platform’s excitation and detection capabilities have been further leveraged to meet the high- content needs of researchers interested in up to 23 parameters of cellular analytical measurements.
The system's ease of use enables many more researchers to use the system. In addition, labs which typically used shared resources for such complex experiments can now consider accelerating their work with their own benchtop system.
Minimises light loss, so that even dim populations can easily be detected. It can measure as low as ten molecules of fluorescence and measure particles as small as 0.2 micrometers in size.
Researchers can measure two scatter parameters in addition to up to 19 fluorescent markers with five lasers; and up to 21 fluorescent markers when six lasers are activated. The patent-pending optical design of the system optimises excitation and light collection efficiency from up to six lasers (375, 405, 488, 561, 638, and 808 nm).
A Wavelength Division Multiplexing (WDM) detection module (also patent-pending) features a bandpass-only design that enables various configurations from four to 21 colors, and contains the full complement of repositionable optical filters.
A new version of Beckman Coulter’s CytoFLEX acquisition software, CytExpert Version 2.0, has also been introduced and is available on the new CytoFLEX LX series. The software’s workflow and novel tools simplify setting up complex flow cytometric applications. By means of a novel compensation library, users are easily able to set up high colour applications, whether they are in the lab or working remotely.
With CytExpert Version 2.0, researchers can compare and reproduce experimental data over time and across multiple systems. Other features ideal for high throughput labs include microtiter plate-based analytics and the ability to interface with front-end automation systems, such as the Beckman Coulter’s new Biomek i-Series liquid handling platform. The software also provides tools facilitating compliance with 21CFR11 regulations such as user management, audit trails, and electronic signatures.
“This heralds a new era for Beckman Coulter Flow Cytometry as we expand on the overwhelming success of the CytoFLEX technology,” said Sharlene Wright, Global Marketing Director at Beckman Coulter Flow Cytometry.
WWith the launch of the CytoFLEX LX, Beckman Coulter can now extend the detection capabilities of this revolutionary technology to the high complexity research market. The LX opens up the opportunity to empower more of the research flow community by offering access to compact, high complexity systems that deliver high quality, high content multiparametric data.”