Beckman Coulter are to host a webinar on the biophysical techniques, such as PALS and Raman spectroscopy, that are suitable forcharacterising the H4-IC31 vaccine formulation.
On August 31st, at 4pm Central European Time, Beckman Coulter will be hosting a webinar on the characterisation of the H4-IC31 vaccine formulation using Phase Analysis Light Scattering (PALS) and Raman spectroscopy techniques.
The aim is to show how vaccine product quality and consistency can be assessed in terms of particle size, zeta potential and conductivity, by these two methods.
PALS is a technique that increases the sensitivity of zeta potential measurement, particularly for samples with low mobility such as those in high salt media and non-aqueous dispersants. It uses the same optical setup as conventional laser Doppler electrophoresis, but a different signal processing method – phase shift – is employed.
Raman spectroscopy is a technique that measures vibrational, rotational, and other low-frequency modes, to provide a structural fingerprint for molecule identification.
Together these techniques provide an excellent way of assessing vaccine quality and consistency. Such a molecule is the H4-IC31 vaccine formulation. A tuberculosis vaccine, H4-IC31 consists of a recombinant fusion protein, H4, combined with a novel synthetic cationic adjuvant, IC31®. The H4 fusion protein consists of two immunogenic mycobacterial antigens, Ag85B and TB10.4 while the IC31® adjuvant is a mixture of KLK, a leucine-rich peptide (KLKL5KLK), and the oligodeoxynucleotide ODN1a.
The webinar will discuss the applicability of the biophysical methods to characterize vaccine components in the final H4-IC31 drug product without desorption and how, because they can differentiate between the IC31® adjuvant and the H4-IC31 vaccine candidate, the methods can provide a control during vaccine formulation.
With speakers, Marina Kirkitadze, Head of Biophysics and Conformation Unit at Sanofi Pasteur Ltd, and associate scientist at Sanofi Pasteur Ltd, Sasmit Deshmukh, demand for this webinar is bound to be great so book now online.
To register for this webinar please click here.
