The Opera Phenix High Content Screening System from PerkinElmer is designed for the most demanding high content applications, such as high-throughput phenotypic screening and assays, using complex disease models such as live cells and microtissues.
PerkinElmer, designers and manufacturers of the Opera® High Content Screening System, have taken over a decade’s worth of experience gained from producing this cutting-edge instrument and applied it to the design of their Opera Phenix High Content Screening System.
The result is what they firmly believe to be the premier confocal solution for today's most demanding high content applications, including phenotypic screening and assays of complex disease models, such as live cells, primary cells and microtissues.
Innovative optical design, with extremely sensitive confocal imaging, permits users to obtain richer information at higher throughput than ever before through simultaneous acquisition with minimised crosstalk.
This is thanks to their proprietary Synchrony Optics, which combine a microlens enhanced Nipkow spinning disk, with dual view confocal optics, to separate fluorescence excitation and emission during simultaneous acquisition. The microlens disk has increased pinhole-to-pinhole distance to further reduce out-of-focus noise in thick samples such as microtissues.
Greater speed and sensitivity is augmented by up to four large format sCMOS cameras and simultaneous acquisition in up to four channels. The sCMOS image sensors deliver low signal-to-background noise with a wide dynamic range and high resolution, making them ideal for sensitive and quantitative measurements at short exposure times.
Custom-designed high NA water immersion objectives capture more photons and provide high image resolution to aid the generation of images of exceptional quality even in thick samples. Precise synchronisation of the excitation laser and camera help to minimise phototoxicity and bleaching during live cell assays. And thanks to the large field of view (290% larger than the original Opera HCS system) more cells are sampled in a single image for even more robust statistical analysis.
With modular design and templates for set-up of acquisition channels and parameters, and an intuitive workflow user-interface to guide you through the entire process, the Opera Phenix HCS System easily permits users to generate high resolution images, in up to four colours, at ultra-high throughput.
