Chromatrap's Native ChIP kit is now fully compatible for mass spectrometry (MS) as a downstream process.
Combining Chromatrap’s unique solid-state ChIP technology with MS enables researchers to obtain high quality data sets for chromatin proteomic profiling and mapping of histone modification patterns.
The Native ChIP (N-ChIP) kit ensures a high yield of DNA so that samples can be analysed by multiple downstream processes. It eliminates the use of magnetic or agarose beads, allowing users to quickly and easily recover ready-to-use, purified chromatin for downstream applications including mass spectroscopy. Coupling MS to ChIP allows researchers to shed light on the proteomic landscape of the extracted chromatin, enabling them to begin to understand how proteins interact with repressed and active regions of the genome and their role in global gene regulation.
Chromatin immunoprecipitation (ChIP) is a technique commonly used for mapping the DNA-protein interactions in cells, which are crucial for gene regulation. The technique enriches DNA fragments to which a specific protein of interest or histone modification is bound. Enriched sites can then be identified by qPCR, hybridisation to a microarray (ChIP-on-chip), or by sequencing. This powerful tool is further enhanced by coupling it directly to mass spectrometry, itself an invaluable and versatile tool for the characterisation of biomolecules.
In Native ChIP, the naturally occurring DNA-protein complexes are preserved in their 'native' state by removing the need to cross-link and fix cells prior to extraction.