Tecan’s Rapid EZ DNA-Seq library preparation kit is designed to offer PCR-free generation of sequencing-ready libraries for next generation sequencing (NGS).
Ideally suited to whole genome sequencing and metagenomics for samples with varied GC content, as well as amplicon sequencing, the automation-friendly kit complements the company’s existing Celero, Rapid and Ovation Ultralow DNA-Seq library preparation solutions.
The Rapid EZ DNA-Seq library preparation kit is an end-to-end solution for PCR-free generation of NGS libraries in around 2.5 hours. It uses enzymatic DNA fragmentation and DimerFree library construction technologies, eliminating the need for optimisation regardless of the input amount. It kit enables an ‘add and incubate’ workflow, making it well suited to applications sensitive to PCR bias or PCR artifacts, such as microbiome and prokaryotic samples, or sequencing of GC- or AT-rich regions of the human genome.
The kit includes all of the reagents needed for library construction, with pre-plated Unique Dual Index (UDI) adaptors to enable flexible multiplexing and detection of ‘index hopping’. The simple, flexible workflow can be easily automated on Tecan’s advanced liquid handling platforms, such as the DreamPrep NGS workstation.
Rasmus Lind, General Manager of Tecan Genomics, said: “Our portfolio of innovative DNA-Seq products uses robust chemistries to simplify library preparation, accelerate processing and improve the quality of sequencing data. With the development of the Rapid EZ kit, we now have solutions for every sample type – from very low to high throughput applications.”
To find out more about the Rapid EZ DNA-Seq kit and Tecan’s full range of library preparation solutions, visit Osprey Suite #10 at AGBT, or go to www.tecangenomics.com
