By providing cleavage and deprotection and removing the evaporation stage of oligonucleotide synthesis, the wavePREP from Analytix aims to save up to 95 per cent of processing time.
Oligos are produced using solid-phase synthesis that offers an elevated yield of purified product. To be used in downstream applications, they must be cleaved from the solid support and the protecting groups removed. Whilst the synthesis process is automated many of the post-processing, cleavage and deprotection (C&D), and evaporation steps are still carried out manually, creating a bottleneck which affects productivity and efficiency.
The wavePREP can be used with up to six deep well plates, multiple small columns, or larger columns up to 700ml. The process uses a gas phase method, and all reaction conditions are accurately controlled to maximise the quality and yield. Most steps are automated, and software controlled, including the lowering of the well plates or columns into the reactor, reagent addition, draining and venting. Automatic loading and unloading of reagents ensure there is no operator exposure to chemicals such as ammonia. The system provides excellent C&D yields, even with tricky protection groups and linkers, and eliminates cross contamination.
For further information please contact us at www.analytix.co.uk/contact or call +44 (0) 191 519 4504
