Study demonstrates benefits of Guava's EasyCyte Plus flow cytometry system for investigating complex interplay of cytokines and immune cells at both the site of infection and in regional lymph nodes
Precise regulation of effector function is critical for mounting a potent, yet specific immune response to a given antigenic challenge.
It has been shown that the microenvironment cytokine content of secondary lymphoid organs at the actual site(s) of antigen presentation exert control over the migration and differentiation of immune cell populations.
Using a multiparametric analysis approach, the application study describes how the effects in culture of all possible combinations of as many as six cytokines on the phenotype and functional capacity of CD4+ T cell isolates derived from human peripheral blood were investigated.
The application study was carried out using the Guava EasyCyte Plus platform, a microcapillary-based cell cytometry system combining the capacity for simultaneous detection of four fluorescent parameters with 96-well high-throughput screening.
Simultaneous four-colour fluorescence detection greatly expands the results obtainable from any one well, simplifying simultaneous monitoring of more complex biological studies such as cytokine production.
Monitoring the interplay of up to four different biological mechanisms simultaneously improves the efficiency of experimental outputs.
Not only does this shorten the number of iterations for any one experiment, it also comes that much closer to mimicking the complex biological responses within the body.
Unlike other flow cytometers the Guava EasyCyte Plus provides automated cell count and viability analyses of a chosen population to quality control cells prior to commencement of complex staining procedures.
Thus preventing wastage of valuable cells and reagents.
The Guava EasyCyte Plus then detects these complex stained cells using its new dedicated multiparametric detection software, Guava ExpressPro.
Data from the application study 'Assessing the regulation of CD4+ T-Cell function by distinct cytokine combinations on the Guava EasyCyte Plus platform' clearly demonstrated that unique combinations of cytokines had profound and highly varied effects on lymphocyte fate and function in vitro.
Guava Personal Cell Analysis (PCA) systems, including the Guava PCA, Guava PCA-96, Guava EasyCyte, Guava EasyCyte Plus and EasyCyte Mini systems, are integrated, fully optimised, microcapillary cytometry systems with embedded absolute cell counting capability.
Used worldwide by the life sciences, biotechnology, and pharmaceutical industries, as well as clinical testing institutions (outside the United States and Europe), Guava's products have broad applications in scientific research and throughout the drug discovery and lead optimisation process, as well as for cell counting and optimisation of commercial biopharmaceutical production.
Guava offers a variety of assays and dedicated software modules for the Guava PCA systems, enhancing the system's overall ease-of-use.
Copies of 'Assessing the regulation of CD4+ T-Cell function by distinct cytokine combinations on the Guava EasyCyte Plus platform' are available from Guava Technologies.
