Rapid Listeria has received Afnor approval to ISO 16140 requirements, and becomes is the first chromogenic medium to be validated for the detection of Listeria spp in environmental and food samples
The new method Rapid Listeria spp method provides the laboratories with a rapid and economical solution for Listeria spp detection, with full results in just 48hrs.
Principle: Rapid Listeria has been specifically developed for the detection of all Listeria species in just 24hrs only after 24hrs of enrichment, from environmental samples and food.
During an inclusivity study, Listeria innocua, L monocytogenes, L welshimeri, L seeligeri, L ivanovii and L grayi presented typical blue-green colonies after 24hrs of incubation.
The chromogenic reaction is based on the specific beta-glucosidase activity of the Listeria.
The selectivity of the medium inhibits most of the interfering flora and the nutritiousness of the medium provides rapid growth and identification of Listeria spp in 24 hrs.
Reading and differentiation are very easy thanks to the good contrast between blue-green Listeria colonies and the whitish agar.
Method: the Rapid Listeria spp detection method requires a single enrichment step in 1/2 Fraser for 24 hrs.
Then 0.1ml of 1/2 Fraser is removed, spread and isolated on Rapid Listeria spp agar, before being incubated for 24hrs.
Key benefits:.
Economical: just one broth and one plate only are required per sample.
Rapid response in after just 24hrs of incubation only for all Listeria species, after enrichment.
Validated high specificity and selectivity.
Easy-to to-read chromogenic reaction.
The Rapid Listeria spp method is Afnor-ISO 16140 validated for the detection of Listeria spp.
