Bio-Rad application note, bulletin 5632, provides information on the ability of ProteoMiner protein enrichment kits to concentrate and enrich low- and medium-abundance proteins for biomarker discovery
Entitled, 'Accessing low-abundance proteins in serum and plasma with a novel, simple enrichment and depletion method', the six-page application note describes how the ProteoMiner protein enrichment kit represents an effective and novel sample preparation method that can concentrate low-abundance proteins and reduce the dynamic range of protein concentrations in a sample.
The paper demonstrates how the use of the ProteoMiner protein enrichment kit in a Seldi- or 2DGE-based proteomics workflow can increase peak or spot counts from plasma and serum samples by 30 to 50%, thereby allowing detection and mass spec identification of medium- and low-abundance proteins.
The product application note (bulletin 5632) is available either from a local Bio-Rad sales office or can be downloaded from the Bio-Rad Laboratories website (linked).
