Trevigen announces the immediate availability of the Cultrex endothelial cell invasion kit for measuring early angiogenesis in vitro.
The Cultrex endothelial cell invasion kit was created in an effort to accelerate the screening process for compounds that influence vascular endothelial cell invasion and migration across the basement membrane.
This kit offers a flexible, standardised, high-throughput format for quantitating the degree to which endothelial cells penetrate a barrier of basement membrane extract (BME), in vitro, in response to chemoattractants and/or inhibiting compounds.
This assay employs a simplified Boyden chamber-like design with a basement membrane coated 8-micron polyethylene terephthalate (PET) membrane.
Sulphoraphane, a naturally occurring cancer chemopreventive agent, is provided as a control for inhibition of in vitro endothelial cell migration/invasion on/through Cultrex Basment membrane extract.
The assay is rapid and detection of cell invasion is quantified using Calcein-AM.
