The complete 2D system from Hoefer includes the IEF100 First-dimension isoelectric focusing unit and the SE900 Second-dimension gel electrophoresis unit
Hoefer has introduced a new two-dimensional electrophoresis system it says that offers the highest focusing voltage available for the first dimension and has an easy-to-use, highly reproducible, second-dimension separation tank.
The IEF100 will help researchers increase the quality and speed of their IEF separations, and the SE900's single tank design ensures leak free runs and the ability to reuse buffer.
The SE900 is also simple to use, utilises minimal parts, and provides efficient cooling.
The IEF100 integrated 12,000V power supply, with its high voltage and current, enables fast run times and provides both Ethernet and RS232 ports for reporting and control.
The first dimension IEF can be run with IPG Strips of any length commercially available up to 24cm.
Up to six IPG strips can be focused at one time and monitored individually.
The SE900 provides second dimension capabilities, and when used with the IEF100, comprises a complete 2D system.
For example, the IEF100 is used to generate first dimension separations; then the focused strips are transferred to an SE900 second-dimension slab gel for the second dimension separation by molecular weight.
The separation tank can hold up to six second dimension gels.
There is also a gel casting option that can be purchased with the SE900.
The gels can thus be cast in a multiple gel caster that produces identical gels to allow for highly reproducible production of self cast gels.
