In 3D culture, cells are grown in a microenvironment of specialised proteins which mimics in vivo conditions and allows the cells to organise into complex structures rather than uniform monolayers
Many scientists are converting from growing cells in a traditional 2D monolayer on plastic surfaces with tissue culture medium to 3D culture.
Further, 3D cultures are grown on extracellular matrix proteins such as basement membrane extract and laminin I, which are known to play a role in modulating cellular response to pharmacological compounds.
Until now, methods to remove cells from these 3D models have either been inefficient or resulted in the degradation of extracellular receptors which adversely affects biochemical analysis.
Trevigen solves these problems with the release of the the first standardised kit for isolating cells from 3D Culture for biochemical analysis.
The Cultrex 3D culture cell harvesting kit is based on a non- enzymatic approach which prevents biochemical degradation during processing and overcomes the problems associated with extracting cells from BME or Laminin I in 3D Culture.
The quality of the harvested cells is ideal for western blotting, RNA and DNA analysis.
Trevigen is a rapidly growing biotechnology company focused on the development of products and technology for cancer research, emphasizing apoptosis, DNA damage and repair, and cancer cell function and behaviour.
Trevigen has been a long-standing provider of quality reagents and kits for researchers investigating programmed cell death and DNA damage and repair.
A logical extension of the focus on cancer research has been the recent development of assays for cancer cell function and behaviour including angiogenesis, cell invasion and tumour formation.
Currently, the product portfolio contains over 500 products categorised into four processes - apoptosis, DNA damage and repair, angiogenesis, and oxidative stress.
3D qualified BME, Laminin I and Collagen I, as well as 3D cell proliferation kits are also available separately from Trevigen.
