PerkinElmer Life and Analytical Sciences announces the release of six new AlphaScreen SureFire assays for the rapid detection of full-length kinase activation in cell lysates and complex sample types
The six new assays can be utilised for measuring kinase-mediated inflammatory and immune responses, as well as cellular growth and cytotoxicity.
PerkinElmer's AlphaScreen SureFire platform allows for detection of activated kinases by immunosandwich capture of endogenous phosphorylated substrate in cell lysates following treatment of cells with inhibitors and/or activators of signaling pathways.
The AlphaScreen SureFire technology may be used in lab-based or drug discovery campaigns and provides a single assay format that is well-suited for use in high-throughput screening (HTS) and lead optimisation studies aimed at identifying novel kinase inhibitors.
It is the only available no-wash cell-based kinase assay.
The six new assays increase the total number of available AlphaScreen SureFire kits to 28.
The new assays are used for detection of inflammation, cell growth and cell death, and include:.
Phospho-Caspase 9 (Ser196).
Phospho-NF-kB p65 (Ser536).
Phospho-mTOR (Ser2481).
Phospho-IkBa (Ser32/Ser36).
Phospho-IKKa (Ser176/Ser180).
Phospho-IKKb (Ser177/Ser181).
"We are delighted to announce the release of the six new assays for the AlphaScreen SureFire platform, to assist researchers in maximising their high-throughput and secondary screening," said Richard Eglen, president, bio-discovery, PerkinElmer LAS.
"These new additions to the AlphaScreen SureFire line of assays - now totaling 28 - are optimal functional cell-based kinase assays for screening of serine/threonine kinases (STKs), receptor tyrosine kinases (RTKs), growth factor and cytokine receptors, signaling pathways and individual cellular kinase targets".
AlphaScreen technology is ideally suited for developing non-radioactive, homogeneous assays for the direct measurement of phosphorylated peptides and full-length protein substrates, with no wash step required.
AlphaScreen SureFire's ability to measure large protein substrates makes it perfect for application to cellular kinase assays.
The SureFire detection reagents are developed by TGR BioSciences and exclusively distributed by PerkinElmer.
