Bruker Daltronics is to showcase several mass spectrometry products and applications for proteomics at the 7th Human Proteome Organisation (HUPO) World Congress.
MaXis is a high-speed ultra high resolution TOF mass spectrometer platform.
It offers sub-ppm mass accuracy, mass resolution of 40,000-60,000 across a broad mass range, sensitivity and five orders of range, at the spectral rate of 20Hz for MS and MS/MS that is essential for quantitative proteomics.
ETD II is an electron transfer dissociation module for Bruker's HCTUltra high capacity ion trap.
It is based on a negative chemical ionisation source with increased ETD/PTR reactant ion generation, resulting in rapid characterisation of PTMs such as phosphorylations or glycosylations at sub-fmol sensitivity.
HCTUltra's inherent m/z range of greater than 3,000 and its mass resolution that can resolve three+ and four+ charge states of peptides on the fly at full analytical speed means ETD/PTR on the HCTUltra can be used for up to 12 kDa proteins and beyond.
The MALDI-TDS top-down sequencing (TDS) solution provides information from undigested proteins in pharmaceutical and biotechnology applications, including C- and N- terminal assignments, even in the case of N-terminally blocked proteins.
MALDI-TDS is available on Bruker's Ultraflex III TOF/TOF system for the characterisation of PEGylation sites and other protein modifications of vital importance in fields such as recombinant protein production, biologics drug development and quality control.
Bruker's SmartBeam is the enabling technology for MALDI-TDS, complemented by BioTools 3.2 top-down bioinformatics.
The high sensitivity SmartBeam MALDI ion funnel source has been coupled with the ultra-high mass resolution apex-ultra FTMS for MALDI small molecule tissue imaging, including drugs and metabolites from animals dosed at therapeutic levels.
With mass resolution greater than 900,000 (at seven Tesla), the cluttered spectra produced in small molecule MALDI imaging of tissue samples are readily resolved, allowing MALDI-MS imaging of small molecules without the requirement to use MS/MS (SRM) for chemical background suppression.
The high mass accuracy combined with Bruker's SmartFormula software automatically provides sum formula determination for detected molecules.
EASY-nLC is a Bruker-supported nanoscale LC system with 1D and 2D separation capabilities.
Due to the true split-free gradient mixing it avoids cumbersome maintenance, and guarantees robust, steady and reliable operation.
EASY-nLC reduces total solvent consumption and enables fast separations with peak widths less than five sec.
The control by Bruker's Hystar software supports all proteomics applications on the maXis, micrOTOF-Q II, HCT ion trap, Flex MALDI and apex FTMS instrument series for complete, turnkey LC/MS/MS solutions.
The Bruker mass spectrometry products and applications for proteomics will be presented throughout the HUPO conference with more than 30 scientific contributions.
