Trevigen has announced the HT fluorescent homogeneous poly(ADP-ribose) polymerase (PARP) inhibition assay.
PARP, through ribosylation of nuclear proteins, converts DNA damage into intracellular signals that actuate either DNA repair or cell death.
The development of inhibitors to PARP is of ever increasing interest in the drug discovery community since they can be used as modulators of DNA repair mediated resistance to cytotoxic cancer therapeutics.
Trevigen's HT fluorescent homogeneous PARP inhibition assay is based on the consumption of NAD+ in the PARP ribosylation reaction where NAD+ not consumed by PARP is subsequently measured by a cycling reaction in which a highly fluorescent molecule is produced; therefore an increase in signal positively correlates to increased PARP inhibition.
The 96 test assay requires 1.5h to complete.
It requires no wash steps, and detects as little as 10 per cent inhibition of PARP activity.
Trevigen said the assay is ideal for the determination of IC 50 values for PARP inhibitors.
