Meridian Life Science has announced its Elisa kit for the detection of protein A leakage during antibody purification.
Each kit includes two protocols; one for samples containing immunoglobulin and one for samples without immunoglobulin.
Analysing the presence of staphylococcus protein A (SPA) in samples containing immunoglobulin involves two problems.
The Fc-reactivity of SPA with immunoglobulin of most animal species makes the specific detection by immunoassay of SPA difficult due to the general affinity between SPA and immunoglobulin Fc.
In addition, in samples containing mammalian immunoglobulin, the immunological active epitopes of SPA are normally blocked by the non-specific binding of immunoglobulin.
Meridian said its Elisa kit for protein A detection has been designed to overcome these problems by using chicken anti-SPA immunoglobulin, as chicken antibodies do not have Fc-reactivity to SPA.
