Beckman Coulter has introduced an enhanced Chiral Methods Development Kit for CE (capillary electrophoresis) analysis on the company's P/Ace MDQ System.
The new kit features highly sulphated cyclodextrins (HSCDs) - a family of three chiral reagents that deliver excellent performance in the separation of most neutral, basic and weakly acidic compounds of pharmaceutical and biological interest.
The Chiral Methods Development Kit includes a user guide with a complete protocol.
CE uses significantly less sample and reagent than HPLC for chiral separations, while providing broad selectivity and fast results.
Documented studies of more than 200 compounds have demonstrated that this protocol provided baseline enantiomer separation for more than 90 per cent of the analytes.
Beckman Coulter said that its Chiral Methods Development Kit minimises method development time to as little as half a day, compared with two days or more for HPLC.
The run time itself is also typically much faster.
CE uses no chlorinated solvents or toxic reagents, so disposal costs are minimised.
Reagent volumes have also been changed to meet the evolving needs of the user.
The original 100ml glass bottles have been replaced with 30ml plastic bottles.
The development kit contains three 5ml bottles (HS-alpha-CD, HS-beta-CE and HS-gamma-CD).
Beckman Coulter's complete package for the separation of enantiomers includes the P/Ace MDQ configured with a photo diode array detector, sample storage module and 32 Karat Software.
