Anaspec has brought out a selection of fluorescent Ca2+ indicators, including indicators that are UV-excitable and visible-light excitable.
Fura-2 and Indo-1 are the most commonly used UV-excitable calcium indicators.
Fura-2 is excitation-ratioable while Indo-1 is emission-ratioable.
Fluo-3 and Rhod-2 are the most commonly used visible-light excitable calcium indicators.
Fluo-3 indicators are widely used in flow cytometry and confocal laser-scanning microscopy.
Fluo-3 AM has recently been used in cell-based HTS assays for functional GPCR assays.
The long-wavelength Ca2+ indicators, Rhod-2 and X-rhod-1, are alternatives to Fluo-3 for experiments in cells and tissues with high autofluorescence.
