The Decyder 2D 7.0 is a software package for analysing 2-D fluorescence difference gel electrophoresis (2-D DIGE) experiments.
2-D DIGE detects and quantitates biological differences in protein abundance between different samples.
Decyder 2D 7.0 applies a gel comparison method that offers reliable data and analysis for 2-D DIGE experiments.
Decyder 2D 7.0's co-detection algorithm means it introduces fewer false positives/negatives.
The software also reduces hands-on time from days to minutes, with minimal user-to-user variation.
Advanced statistical analysis and visualisation tools allow deep understanding of the contribution of variation to the data.
Linking of data from two or more datasets increases sample population to allow better differentiation between real and experimental variation.
Decyder 2D 7.0 is a key component of the Ettan DIGE system.
Protein mixtures are labelled prior to electrophoresis with size and charge-matched spectrally resolvable Cy Dye fluors, allowing the simultaneous separation and comparative analysis of up to three samples on a single 2-D gel.
Following 2-D electrophoresis, the results can be scanned with the Typhoon Variable Mode Imager or Ettan DIGE Imager.
Decyder Differential Analysis Software automatically locates and analyses protein spots.
As the system ensures each protein spot has its own internal standard, gel variation issues are eliminated, increasing accuracy and reproducibility and reducing the number of gels that need to be run.
