GE Healthcare has introduced a mammalian protein extraction buffer and yeast protein extraction buffer kit.
These have been designed for the efficient extraction of soluble protein from mammalian cultured cells and yeast cells.
The mammalian protein extraction buffer and yeast extraction buffer kit are mild, non-denaturing buffer compositions, delivering quality protein lysate compatible with most downstream applications without the need for mechanical cell lysis.
Both products give reproducible, consistent results with maintained biological activity and address the need for simple and versatile sample preparation methods for protein extraction.
The mammalian protein extraction buffer is a 500ml solution designed for extraction of total soluble proteins from mammalian cultured cells.
The buffer can be used for both cell suspensions and adherent cells.
The buffer is compatible with most downstream applications, including chromatography, electrophoresis, enzyme assays, kinase assays and immunoassays.
The yeast protein extraction buffer kit is designed for extraction of soluble proteins from yeast cells, and is suitable for preparation of approximately 10ml yeast cell pellet suspension, either as a single preparation or as multiple, smaller preps.
The kit eliminates the need for glass beads, and is a proprietary improvement on the Zymolyase-based method for spheroplast preparation and extraction of soluble proteins from yeast cells.
The buffer kit is compatible with any downstream application, including enzyme assays, chromatography and gel electrophoresis.
