GE Healthcare has introduced 2D protein extraction buffer, a series of six buffers for efficient and reproducible extraction of high-quality protein lysates with high yield.
Resulting protein lysates are suitable for applications such as 2D gel analysis and 1D electrophoresis, or for rehydrating IPG strips prior to 2D electrophoresis.
The six new buffers (2D protein extraction buffer-I to -VI) are modifications of well-studied protein solubilisers, and are available as a dry powder which is rehydrated with the provided diluent.
The optimal buffer depends on the nature of the sample.
The 2D protein extraction buffer trial kit is also available for evaluation of each extraction buffer, enabling optimisation of total protein extraction.
The dry powder formulation eliminates problems associated with carbamylation, which can occur in solutions containing urea and may alter protein charge, producing spot artifacts on 2D spot maps.
Each package contains the reagents necessary to prepare 50ml buffer, and the 2D protein extraction buffer trial kit includes reagents for preparing 10ml of each of the six buffers.
