MDS Analytical Technologies has announced Metamorph 7.6 software, which features application modules for three-dimensional motion tracking and micronuclei detection.
This release of Metamorph, compatible with MDS Analytical's line of cellular imaging products, also incorporates a number of other enhancements, including support for enhanced deconvolution with Optigrid structured illumination microscopy (SIM), and automated slide handling.
Multidimensional motion analysis (motion analysis in 3D) will allow users to easily select multiple objects, and track their position and velocity within the sample volume.
Competing tracking systems require users to manually identify the original set of objects for tracking and prevents automated tracking, a feature that is particularly useful with large numbers of similar objects.
Another enhancement to Metamorph 7.6 is the micronuclei application module, which allows users to find, evaluate and quantitate micronuclei in any nucleated cell.
This is an improvement on competing application modules that only allow users to find micronuclei in a multi-nucleated cell.
Using additional probes, the module also enables users to differentiate between mitotic, apoptotic, and necrotic cells.
The Optigrid SIM is now fully controlled, using Metamorph software via a USB connection, which allows for simple system integration.
The structured illumination process returns a strong signal wherever focus is sharp and a weak signal where focus is soft.
A series of optical sections taken through a sample (z-stack) can then be combined to create a haze-free ultra-sharp composite image.
Image stacks can also be used to produce 3D reconstructions using post-processing software.
Along with Metamorph 7.6, the Ludl Electronic Products slide handling system offers scientists an easily expandable system for microscope automation.
A single arm pulls the slide from the cassette to the stage and then returns it with an opposite pushing motion.
As the precision XY stage scans the slide, the transfer mechanism is completely clear of any interference with normal microscope function, which helps make the system suitable for low-volume repetitive analysis, and review applications.
