Thermo Fisher Scientific, a specialist in serving science, has announced that its Macherey-Nagel Protino Glutathione Agarose 4B kit is now available to UK scientists.
Protino Glutathione Agarose 4B and Protino GST/4B columns enable fast and convenient purification of recombinant Glutathione-S-Transferase (GST)-tagged proteins from whole-cell extracts by affinity chromatography.
The binding of GST-tagged proteins using Protino Glutathione Agarose 4B is highly specific, based on the selective enzyme-substrate interaction between the GST tag of the recombinant protein and the immobilised glutathione of the affinity resin.
Thus, only one chromatographic step leads to a high degree of purification.
There is high reproducibility between FPLC runs and highly consistent upscaling, from 1ml to 10ml bed volume.
This matrix is available in 10ml or 100ml volumes (settled beads) or pre-packed in ready-to-use 1ml Protino GST/4B columns and is suitable for the purification of small proteins, large protein complexes and proteins with low expression rates.
