Fluxion Biosciences has introduced the Ionflux-HT and Ionflux-16 automated patch clamp systems, designed to offer gains in throughput, workflow simplicity and affordability to ion channel research.
These systems combine Fluxion's microfluidic technology with integrated electronics to automate the traditional patch clamp assay.
Suitable applications include primary/secondary screening, lead optimisation and toxicology screening.
The Ionflux-HT system includes 64 amplifiers and delivers between 8,000 and 10,000 assays per day at a cost of just 30 cents (20 pence) per data point using 384-well plates.
The Ionflux-16 provides all the features of the HT system with 16 amplifiers, utilising a 96-well plate format.
Delivering 2,000 data points per day, the Ionflux-16 system is offered at a cost that addresses the needs of many researchers who cannot afford the high price tag of current automated patch clamp systems.
Both the Ionflux-HT and Ionflux-16 systems use standard well-plate formats with integrated microfluidic channels for cell patching and compound addition.
The systems employ a familiar plate reader format for simplicity and integration with existing automated liquid handlers.
The Ionflux technology automates cell manipulation, trapping, sealing, whole-cell formation and precise voltage protocols.
Voltage clamping occurs continuously during all fluidic operations.
Automated compound addition during the voltage protocol provides delivery to the cells in 50 milliseconds, making the system suitable for ligand-gated channels.
