Cybio and Axxam have developed, installed and validated a screening station to be used by Axxam in running hit discovery campaigns with automated luminescence and fluorescence screening systems.
The new workstation, which will run in parallel to another Cybio workstation, is a customised, multi-functional automation platform that allows flexible adaptation to different assay conditions using high-density 384 plates.
A central Cybi-Well vario high parallel pipetting system unites two major working areas.
Cooled storage system, peeler, sealer and plate heating stations frame the area for compound management.
The screening area consists of a pipetting system, a dispenser, a washer, high-capacity stacking and incubation devices with lid handling options, two readers, a Fliprtetra (Molecular Device) and a PherastarPlus (BMG Labtech).
They can be used to acquire fluorescent or luminescent readouts, both in cell-based or biochemical assays.
All modules are integrated by a Staubli six-axis robot and a Kinedx four-axis robot that ensure high speed and precision in plate transfers.
The system as a whole is controlled by the Cybio Scheduler software that manages automatic parallelisation of processes, consistent system utilisation and time uniformity across screening.
This results in high consistency in assay performance and in a throughput that ranges from 35,000 for long acquisition-time readings to 47,000 test points per day.
Axxam will benefit from broader access to automated high-throughput screening and will consolidate its position as an important discovery partner for target-based screening discovery programmes.
