Amsbio, a provider of products and custom services for life-sciences research, has launched its Iso-Gold rapid mouse monoclonal isotyping kit.
Iso-Gold is a rapid-format isotyping kit that reduces the labour of the traditional Elisa protocol from a 1.5-day procedure to a five-minute assay.
Determining the class and subclass of a monoclonal antibody is valuable in selecting the best immunoglobulin purification method, according to the company.
The Iso-Gold kit is a rapid lateral flow assay, with a sensitivity of 10ng/ml that can be run on both tissue culture supernatant and mouse ascites fluid.
Unlike Elisa-based isotyping kits that require considerable sample preparation and kit development, Iso-Gold is claimed to be very easy to use.
In a single step, the diluted antibody sample is added into the loading well of the cassette.
In less than five minutes, the Iso-Gold kit will provide a colour readout of the mouse monoclonal antibody isotope (IgG1, IgG2a, IgG2b, IgG3, IgA or IgM).
The test is also available with kappa and lambda light chain determination.
Iso-Gold kits have a long shelf life - they are proven stable at room temperature for more than one year.
The proprietary kit is also specific - the assay does not cross react with fetal bovine serum (FBS).
A data sheet describing the Iso-Gold kits can be downloaded from the company's website.
Amsbio conducts research in a number of areas, including apoptosis, cell invasion and migration, cell signalling, DNA damage, electrophoresis, glycobiology, posttranslational modification and stem cell biology.
