Thermo Fisher Scientific has launched two mass spectrometers, the Thermo Scientific LTQ Velos and LTQ Orbitrap Velos systems, at Achema 2009 in Frankfurt, Germany, from 11-15 May.
The LTQ Velos features a dual pressure trap design and API source, making it a fast and sensitive ion trap mass spectrometer.
The dual pressure trap technology features two discrete pressure regions that allow the decoupling of ion manipulation and detection.
This allows the use of optimal pressures during analysis, increasing scan speed and resolution.
The LTQ Orbitrap Velos combines the Orbitrap mass analyser, a higher-energy collisional dissociation (HCD) cell and dual pressure trap technology to routinely deliver ultra-high resolution and accurate mass data.
The speed of LTQ Velos-based mass spectrometers is ideal for coupling to U-HPLC systems, enabling researchers to identify more compounds in less time.
The data quality and sensitivity of the LTQ Velos make it ideal for the analysis of complex mixtures, such as the identification of low-level proteins and structural elucidation of metabolites in biological matrices.
For proteomics applications, the improvements in speed and sensitivity provide greater coverage in the analysis of complex peptide mixtures, increasing the confidence of protein identification at low sample levels.
The multiple fragmentation techniques available on the LTQ Velos allow more confident sequence assignment and post-translational modification (PTM) identification.
Faster scan rates also reduce cycle times by up to 50 per cent, doubling the number of proteins and peptides identified.
For metabolism applications, the dual pressure trap technology increases fragmentation efficiencies, resulting in faster, more confident structural elucidation.
The improved speed and sensitivity combines with MSn capabilities, maximising throughput while maintaining the superior data quality required for the identification and quantitation of multiple co-eluting compounds.
The LTQ Velos can be upgraded to the LTQ Orbitrap Velos, allowing laboratories to extend their initial investment to include accurate mass and ultra-high resolution capabilities, without compromising sensitivity and speed of analysis.
The LTQ Orbitrap Velos combines the mass accuracy and ultra-high resolution of the Orbitrap mass analyser with the increased sensitivity and improved cycle time of the LTQ Velos to deliver high- performance hybrid mass spectrometry.
The high mass accuracy of the LTQ Orbitrap Velos increases the speed and confidence of protein identification in complex samples by minimising false positives.
The ultra-high resolution delivers certainty in analytical results by permitting molecular weight determination for intact proteins and in-depth analysis of isobaric species.
For proteomics researchers, these capabilities allow the identification of more proteins, with increased sequence coverage and higher confidence.
The HCD cell in the LTQ Orbitrap Velos is more efficient, resulting in improved quantitation of isobarically-labelled peptides, such as applications requiring tandem mass tags (TMT).
Electron transfer dissociation (ETD) generates complementary information for highly sensitive PTM analyses and de novo sequencing.
For metabolism applications, the LTQ Orbitrap Velos provides researchers with high-resolution accurate mass data for more confident structural elucidation.
