Oxoid has announced the availability of two chromogenic media in the Brilliance resistant screening agar range.
Brilliance ESBL Agar and Brilliance VRE Agar can be used as screening tests to rapidly identify patients colonised with problematic Extended Spectrum Beta-Lactamase (ESBL) producing organisms and vancomycin-resistant enterococci (VRE), allowing appropriate infection control and treatment to commence sooner for the best possible patient outcome.
Brilliance ESBL Agar provides presumptive identification of ESBL-producing E coli and the Klebsiella, Enterobacter, Serratia and Citrobacter (KESC) group, direct from clinical samples.
Supplied in ready-to-use plates, results are available in 24 hours.
The semi-opaque background of the medium contrasts with the brightly coloured colonies and allows clear and easy differentiation of E coli (blue colonies) and the KESC group (green colonies).
The inclusion of Cefpodoxime, a well recognised marker for ESBL mediated resistance, inhibits most non-ESBL Enterobacteriaceae.
Inhibition of AmpCs reduces incidence of false-positive results compared to traditional media minimising confirmatory testing.
Brilliance ESBL Agar has recently been selected by MOSAR (the FP6 EC funded project co-ordinated by the French Inserm) for use in a pioneering European ESBL prevalence study.
Brilliance VRE Agar is a chromogenic screening plate for the detection of VRE.
Following direct inoculation from faecal sample, swab, isolate or suspension, the medium provides presumptive identification of Enterococcus faecium and Enterococcus faecalis within 24 hours.
Differentiation of vancomycin resistant E faecium from E faecalis is achieved through the inclusion of two chromogens that are targeted by specific enzymes.
The action of these enzymes on the chromogens results in a build-up of colour within the colony.
The colour produced depends on which enzymes the organisms possess.
Additional antibiotics are present in combination with vancomycin, to suppress the growth of competing flora including E gallinarum and E casseliflavus, both of which are intrinsically resistant to vancomycin.
Early presumptive identification of ESBL-producing organisms and VRE permits appropriate treatment and infection control procedures to be adopted sooner, improving both treatment outcomes and the effectiveness of infection control measures.
