Amsbio has announced further additions to its enzyme assays portfolio, including new direct activity assay kits for methyltransferases - PRMT1, 3, 5 and Set 7/9.
The kits have been developed to enable the measurement of enzyme activity using purified enzymes or enzyme-containing extracts.
Each direct activity assay kit is supplied in a 96-well plate pre-coated with peptide substrates, an antibody against the specific methylated target, a secondary Europium-labelled antibody, S-adenosylmethionine, methyltransferase assay buffer, and purified enzyme for 100 enzyme reactions.
The key to the direct activity assay kits are highly specific antibodies that recognise methylated residues of the different substrates and only a few steps are required for detection of methyltransferase activity.
First, enzyme and co-substrate are incubated in the substrate-coated microtitre wells.
Next, primary antibody is bound to the methylated substrate.
Finally, the plates are treated with a Europium-labelled secondary antibody followed by addition of the enhancer to produce fluorescence at 620nm.
Fluorescence can then be measured using a fluorescence reader equipped for the measurement of time-resolved fluorescence.
