Thermo Fisher Scientific has launched the 'QPCR Optimisation and Troubleshooting Guide', an educational tool for researchers.
The guide is said to be a useful resource for those beginning to develop a QPCR assay, those looking to optimise a QPCR assay or those wanting to understand why they are getting inconsistent results.
This guide aims to educate researchers that are new to the technique to better understand the science of real-time PCR.
It should enable them to develop their skills in troubleshooting and optimisation.
The Optimisation section of the guide covers parameters such as choice of plate, amplicon size, primer design, and it makes recommendations to optimise QPCR performance.
In the troubleshooting section, problems, possible causes and remedial actions are addressed.
