Porvair Sciences has announced a technical report that evaluates well-to-well crosstalk in commercially available microplates used for luminescence measurements.
The technical report compares two different manufacturer's solid white polystyrene shallow well microplates with Porvair's Krystal 2000 black-and-white design, which combines white individual wells with a solid black plate matrix.
A luminescent assay using Firefly luciferase was used for the comparison.
The reported experimental results demonstrate that the Krystal 2000 black-and-white plate offers benefits for the determination of low-level luciferase-based assays in screening and drug development.
The combination of effective quenching by carbon black and increased reflectivity from titanium dioxide brightener is said to yield an improved signal-to-noise ratio and better intra-plate dynamic range, giving screeners the opportunity to screen for weaker hits, at lower detection levels or with reduced concentrations of reagents.
Designed to conform to the standard 96-well microplate format - Porvair's Krystal 2000 black-and-white plate is fully compatible with all commercially available top-reading luminometers, multimode readers, robotic sample processors and automated liquid handling systems.
