AnaSpec has introduced the HiLyte Fluor 405, a bright blue fluorescent probe from the HiLyte Fluor series of fluorescent labelling dyes.
They are optimised for maximum brightness when conjugated with macromolecules.
Available in a variety of reactive forms, such as amine-reactive, thiol-reactive and amine-containing derivatives, HiLyte Fluor 405 allows for easy conjugation to biopolymers.
HiLyte Fluor 405 has spectral characteristics similar to those of Alexa Fluor 405 (Invitrogen) and DyLight Fluor 405 (Pierce) dyes with excitation and emission wavelength at 404/428nm in PBS buffer (pH 7.4).
Conjugates of HiLyte Fluor 405 are well excited by the 407nm spectral line of the Krypton laser and the 408nm line of the violet laser diode.
HiLyte Fluor 405 conjugates, whether conjugated to primary tag, secondary antibodies or streptavidin, are prepared in an optimised fluorophore/protein labeling ratio to ensure high fluorescent signal and uncompromised biological function.
Fluorescence signal can be observed at Ex/Em=404/428nm.
