Agilent Technologies has announced availability of the Affinityscript One-Step RT-PCR Kit for high-throughput RT-PCR in a one-step reaction.
The kit is suitable for rapid, one-step setup for RT-PCR and the simple format eliminates the potential for contamination when preparing multiple or high-throughput reactions.
The kit targets researchers performing end-point RT-PCR reactions and provides high product yields and fidelity for applications such as cloning, gene detection and quantification.
The kit consists of Affinityscript RT and Herculase II Fusion DNA polymerase enabling multi-temperature RT, with a robust amplification step to generate higher yields of cDNA with amplicons ranging from 500bp to 9.6kb.
Affinityscript RT can reverse transcribe RNA with high yield over a 37-55C temperature range for first-strand cDNA synthesis.
The use of Affinityscript RT enables cDNA synthesis across an incubation-temperature range up to 55C, which enables reverse transcription though RNA secondary structure, including difficult and GC-rich targets, enhancing the amplification of difficult RT-PCR targets and ensuring high cDNA-synthesis yields from all RNA targets.
By combining the Affinityscript with Herculase II Fusion DNA polymerase, researchers obtain superior yields with equivalent fidelity to Pfu DNA polymerase (6X higher fidelity than Taq), but faster cycling time.
