Beckman Coulter has introduced advanced isoelectric-focusing (IEF) technology, featuring a peptide-based PL marker kit for the charge-heterogeneity analysis of biotherapeutics.
The calibrators are packaged as a set containing markers for PL 4.1, 5.5, 7.0, 9.5 and 10.0, each in sufficient quantity for 100 tests.
Based on capillary-electrophoresis methods, this solution enables biopharmaceutical researchers and technicians to easily implement quantitative isoelectric focusing in a QC environment.
Beckman Coulter has taken a holistic look at capillary-isoelectric focusing (cIEF) and optimised system performance for those parameters that are critical for quantitative reproducibility.
New methods address gradient stability and protein solubility, and the new synthetic PL markers are designed to achieve the highest levels of precision in PL identification and isoform quantitation.
Mark Lies, product manager at Beckman Coulter, said: 'With intermediate precision for calculated PL at less than 0.1 per cent RSD and major isoform quantitation at less than two per cent RSD, the kit allows biotechnology companies to more easily implement cIEF in their QC operations.
'We've worked directly with biopharmaceutical companies to develop universal cIEF methods that cover a broad range of therapeutic MAbs,' he added.
These methods and PL markers were developed for use on Beckman Coulter's PA 800 and PA 800 plus-capillary electrophoresis systems.
Application bulletins detailing the validated cIEF methods and protocols are available for download at the Beckman Coulter capillary-electrophoresis website.
