Phenomenex has announced a web-based tool that optimises customer HPLC methods for use with the company's Kinetex core-shell columns.
The specific benefits to be gained by switching from fully porous particle columns are also calculated.
With the Kinetex Calculator, the researchers input their method running conditions and details regarding their current chromatographic results.
In less than one minute, the calculator delivers an optimised method with the recommended Kinetex column and optimal run conditions for the user's system, whether it's HPLC or UHPLC.
The calculator also provides expected backpressures, efficiency gains, solvent reduction, time savings and resolution improvements.
The Kinetex calculator can process details of isocratic and gradient methods.
Kinetex enables chromatographers to get performance comparable to sub-2um columns on existing LC instruments.
With Kinetex, researchers can achieve UHPLC results on any LC system, completing chromatographic methods in a fraction of the time previously required, without sacrificing performance.
Initially, Kinetex core-shell columns are available in two particle sizes - 2.6- and 1.7um - and three chemistries - C18, PFP and HILIC.
Kinetex 2.6um columns deliver separation efficiencies that match or exceed the performance of traditional fully porous sub-2um columns.
The Kinetex 1.7um is said to be the first ever sub-2um core-shell technology and delivers efficiency gains of 15 to 20 per cent over traditional fully porous sub-2um columns.
Method-transfer challenges are eliminated with Kinetex.
Methods developed with 2.6 or 1.7um Kinetex columns are scalable and can be transferred to any LC system using the 2.6um column, regardless of the instrument's pressure capability.
