UVP has released a white paper that defines the technique for counting multiple colour bacteria.
The Colonydoc-It Imaging Station captures colour-colony images, then differentiates between multiple colours when counting the colonies.
The white paper describes the materials and methods used for differentiating between b-glucuronidase positive (blue) bacteria and negative (yellow) bacteria.
The results generated show detailed counts of each colour and percentage of the population determined to be positive in b-glucuronidase.
The station features: integrated high-resolution digital colour camera; several light sources for illuminating a range of stained media; use of pour, spread and spiral plates and filters with sizes from 33 to 150mm; capture colony sizes as small as 0.08mm; count colonies automatically and generate detailed statistics; doors create a darkroom environment; and GFP fluorescence with Epi blue light and optional GFP filter.
The Colonydoc-It Station allows time-lapse counting and applications such as microbiology, quality control, environmental and pharmaceutical applications.