Olympus has released three high-performance TIRF (total internal reflection fluorescence) objectives.
The 150x ultra-magnification objective offers a numerical aperture (NA) of 1.45 for optimal magnification, resolution, light-gathering ability and imaging performance.
New 60x and 100x oil immersion objectives have NAs of 1.49.
In all, Olympus now offers five specifically designed TIRF objectives.
The 150x UAPON NA 1.45 objective is optimised for single-molecule TIRF applications.
It is said to deliver the high magnification and low background noise necessary for visualising challenging specimens.
The objective delivers crisp images from 340nm ultraviolet (UV) to near-infrared wavelengths and comes with a correction collar to help compensate for aberrations caused by coverglass thickness or variations in immersion media temperatures.
The 100x UAPON NA 1.49 objective has also been enhanced with a correction collar and updated to include near-UV performance.
The 60x APON NA 1.49 provides what are claimed to be the brightest high-resolution TIRF images available, thanks to its improved point spread function.
All Olympus TIRF objectives can take full advantage of the recently introduced Olympus Cell TIRF illuminator, which offers four-colour, simultaneous, fully motorised control of TIRF excitation along with ease of use.
