Amsbio has introduced an anti-DDK monoclonal antibody for the detection of the DYKDDDDK epitope - the same protein sequence as Flag-tag.
Validated for western blot, immunoprecipitation, immunofluorescence and flow cytometry applications, the new 4C5 anti-DDK antibody offers high sensitivity and specificity.
The 4C5 enables researchers to detect proteins with low expression levels and purify recombinant proteins with high purities and yields.
To date, the antibody has been used to validate the expression of more than 15,000 mammalian recombinant proteins, purify more than 5,000 human recombinant proteins from HEK293 cells and purify immunogens under native conditions for the development of antibodies.
Flag-tag is a polypeptide protein tag that can be added to a protein using recombinant DNA technology.
It can be used in many different assays that require recognition by an antibody.
If there is no antibody against the studied protein, adding a Flag-tag to this protein allows the user to follow the protein with an antibody against the Flag sequence.
