Amsbio has announced a range of ready-to-use lentivirus supernatant products suitable for many kinds of gene delivery applications.
These include mammalian protein expression, stable cell-line construction, cell signal pathway localisation and stem-cell research.
Prepared using proprietary protocols to integrate a real-time fluorescence monitoring tag in the system, each 200ml vial of Amsbio lentivirus supernatant contains a high titre of highly transducible lentivirus (1 x 10 e7 IFU/ml).
Each vial of lentiviral supernatant particles contains a fully sequence-verified target, ready for transduction into any mammalian cells.
All Amsbio lentivirus supernatant products are said to be easy and safe to use - users can simply add 50ul into the cultured cells and will be able to confirm the specific target's expression under a fluorescent microscope after 48-72 hours.
The lentiviral system is a gene delivery tool using lentivectors for gene expression or knockdown.
Lentivirus can effectively transduce both dividing and non-dividing mammalian cells, and integrate into the host genome, allowing stable long-term, high-level gene expression both in vivo and in vitro.
Unlike traditional retroviral system, Amsbio lentivirus is much more actively imported into the nuclei of non-dividing cells and stably integrated into the host cell's genome independent of cell cycle.
Although adenovirus is also able to transduce non-dividing cells, it is only for transient expression because it cannot integrate into host cell's genome.
