Millipore has introduced a line of premixed Western blot detection reagents.
Luminata horseradish peroxidase (HRP) substrates offer high sensitivity and chemiluminescent detection.
According to the company, Western blotting has been a routine protein detection method for nearly three decades.
During that time, the final steps have not changed much - most chemiluminescent protocols still rely on an unstable, two-part detection reagent that is mixed just before use for greater sensitivity.
Luminata HRP substrates eliminate that extra mixing step.
Luminata substrates provide a premixed format that speeds up protein detection and reduces cross contamination among multiple reagent bottles.
The Luminata range includes three different sensitivities that, combined with low background signals, enable the detection of protein concentrations down to the femtomole range without enhancers or special buffers.
The reagents are also compatible, complementing Millipore's selection of Western blot reagents and tools, including Immobilon blotting membranes, antibodies, blocking buffers and the Snap ID vacuum-based protein detection system.