Thermo Fisher Scientific has developed the Hypersil Gold HILIC column range to aid the analysis of compounds that are traditionally difficult to retain using conventional C18 columns.
With the ability to retain highly polar and hydrophilic compounds, the columns are available with 1.9, 3 and 5um particle sizes, and are suitable for applications within drug discovery, food testing and environmental analysis.
The company will showcase the Hypersil Gold HILIC columns in Thermo Scientific booth 610 during the HPLC 2010 exhibition in Boston on 19-21 June.
HILIC works by incorporating water in the highly organic mobile phase to form an adsorbed water-rich layer on the polar stationary phase surface, into which analyte molecules partition.
Retention is governed by dipole-dipole interactions and hydrogen-bonding mechanisms.
The highly organic mobile phase contains low salt levels, making Hypersil Gold HILIC columns suitable for use with electrospray mass spectroscopy.