Amsbio has launched a range of expression-ready lentiviral particles that allow easy gene delivery into hard-to-transfect cells, including primary cells, non-dividing cells and cells in suspension.
The lentiviral particles are supplied ready-to-use, eliminating the need for any lipids or transfection reagents.
Amsbio said the range of pre-made lentiviral particles are proven to deliver genes in a highly reproducible and controllable manner for the expression of greater than 250 human proteins, mouse proteins, enzymes and fluorescent markers.
Using proprietary protocols based upon co-expressed red fluorescent protein by a 2A peptide processing (Suretiter) or RSV promoter (inducible system), Amsbio lentiviral particles enable real-time monitoring of protein expression.
The lentiviral particles are well suited for applications in gene delivery for expression or knock-down in hard-to-transfect cell types such as primary cells, stem cells, drug-arrested cells and cells in suspension.
Amsbio lentiviral particles can also be used to cost-effectively create stable cell lines for long-term high constitutive or Tet inducible expression applications in the presence of TetR (a repressor protein).
Furthermore, Amsbio lentiviral particles can also be used in vivo for the generation of transgenic animals and for inducing pluripotent stem cells (IPSC).
Custom lentiviral particles for the expression of customers' gene of interest or shRNA can also be generated.
All Amsbio lentiviral products are easy and safe to use; users simply add the high titre particles (1 x 107 IFU/ml or higher) to cultured cells, and will be able to confirm the specific target's expression under a fluorescent microscope after 48-72 hours.
The lentiviral particles are packaged in culture medium with 10 per cent FBS, in serum-free medium or concentrated in PSB for in vivo use.
