Lab M has added two products to its portfolio of culture media for the isolation of yeasts and moulds from foodstuffs.
The Dichloran Rose Bengal Chloramphenicol Agar (ISO) and Dichloran 18 per cent Glycerol Agar (ISO) media comply fully with the requirements of ISO 21527-1:2008 and ISO 21527-2:2008 respectively - standards that relate to the enumeration of yeasts and moulds in food and animal feeding stuff.
Both products are supplied dehydrated as complete formulations that are ready to prepare without the need for any additional supplementation.
The incorporation of the antifungal agent dichloran in the new media restricts the spreading of mucoraceous fungi and controls the colony size of other genera.
Dichloran Rose Bengal Chloramphenicol Agar (ISO) (DRBC Agar) is designed for the enumeration of viable yeasts and moulds in products with a water activity above 0.95.
It is prepared according to ISO 21527-1:2008 and complies with Bacteriological Analytical Manual (BAM) and American Public Health Association (APHA) methods.
In addition to glucose, dichloran and the anti-bacterial chloramphenicol, the medium incorporates Rose Bengal, which helps limit colony size and is selective against bacteria.
Dichloran 18 per cent Glycerol Agar (DG18 Agar) is for the enumeration of viable osmophilic yeasts and xerophilic moulds in food or animal feed products that have a water activity of 0.95 or less.
The reduced water activity in this medium is achieved through the addition of glycerol at approximately 18 per cent - the importance being that many yeast and moulds require a low water activity to enhance growth and colony development.
Prepared according to ISO 21527-2:2008, DG18 is also and compliant with methods set out in the BAM.
