Trevigen has made available 19 DNA repair gene knockdown cell lines to enable scientists to study the molecular etiology of tumour genomic instability and exploit it in oncology research.
The DNA repair gene knockdown cell lines encompass the Base Excision Repair pathway.
These are target-specific LN428 glioblastoma shRNA lentivirus transduced cells that are rigorously qualified and mycoplasma free.
The percent knockdown levels range from 75-98 per cent as determined by RT-PCR.
Lentiviruses are maintained by puromycin selection.
DNA repair proteins maintain the stability of the genome.
When repair protein function is impaired through mutations, the genome may become unstable, which is a hallmark of solid tumours.