Illumina has announced Truseq sample preparation kits for DNA, RNA and small RNA applications, plus the Truseq Exome Enrichment kit, for use across the company's portfolio of sequencing platforms.
Truseq sequencing reagents provide a streamlined workflow that is economical and scalable, said Illumina.
'The benefits of Truseq are critical for our customers who are routinely generating more than 300Gb per run with their Hiseq systems,' said Christian Henry, general manager of life sciences for Illumina.
Truseq sample preparation kits feature master-mixed reagents and optimised adapter design.
Truseq Exome Enrichment kits, used with Truseq DNA sample prep kits, enable large-scale exome studies using a pre-enrichment pooling approach with plate-based processing of up to 96 samples.
'The RNA sample prep kit allows us to take full advantage of the increased sequencing output of our Illumina instruments,' said Greg May, president and chief operating officer at the National Center for Genomic Resources.
'RNA-seq is now cost competitive with microarrays, allowing the design of reasonably priced, rigorous gene expression experiments with appropriate sample sizes for statistical analysis,' he added.
Truseq solutions provide: coverage of greater than 62Mb of the human exome; a flexible approach for single-end, paired-end and multiplexed sequencing; sequencing-based gene expression studies with richer data sets; and a means for researchers to combine up to 48 samples into a single sequencing lane, profiling the whole spectrum of small RNAs with high sensitivity and specificity.
