GE Healthcare's Membrane Protein Purification Kit is for rapid detergent screening of histidine-tagged membrane proteins for simultaneous evaluation of solubilisation and purification efficiency.
Analysis and evaluation can be performed by a number of methods such as Western blot, gel filtration or light scattering.
The kit contains seven detergents in a ready-to-use solution (purity is greater than or equal to 99 per cent by HPLC analysis), His Mag Sepharose Ni, buffer stock solutions and a protocol that is said to be easy to follow and sufficient for two complete screening experiments.
Integral membrane proteins play major roles in fundamental biological processes and are common targets in the development of pharmaceuticals.
To be studied, these proteins must be dispersed in an aqueous solution.
To avoid protein loss and deactivation during solubilisation and concomitant purification of integral membrane proteins, the choice of detergent is a key factor.
Therefore a detergent screen is often necessary to determine the optimal detergent for each protein and purpose.
His Mag Sepharose Ni are magnetic beads designed for rapid, small-scale purification and screening of histidine-tagged proteins from different sources.
The high-density beads allow rapid capture by magnetic devices, while the visibility of the beads permits collection of the bound histidine-tagged membrane proteins in the screening and purification procedure.
Purification of target membrane proteins for a number of analytical or other downstream applications can be achieved within a few hours.
