Trevigen has released the Culturecoat optimisation system, a 24 insert-well format assay that enables scientists to select the desired degree of cell invasion necessary for a specific project.
The system includes a series of Boyden chambers coated at three distinct concentrations of BME and uncoated inserts to compare invasion to basal migration.
Relative to uncoated inserts cell invasion decreases as the concentration of the BME coating increases.
This assay provides an easy approach to determine the ideal coating density that provides the maximum signal-to-noise ratio and dynamic range required for the customer's study.
Once the desired concentration is determined, inserts coated with that concentration of BME can be ordered.
The system is now available in both 96- and 24-well formats.
With the 24-insert assay researchers have the ability to perform fewer tests and control the number of samples per assay.
The individual inserts also have a larger surface area, making them more practical for sub-cloning invasive cell populations.
