Agilent Automation has created a fully optimised and validated high-throughput target enrichment platform - Sureselect XT - to allow researchers to sequence genomic regions of interest.
Sureselect XT combines next-generation sequencing library preparation and gDNA preparative reagents with the Sureselect target enrichment system.
This is said to add a high level of convenience and productivity to high-performance target enrichment.
Target enrichment allows researchers to sequence just the genomic regions of interest rather than entire genomes.
Combined with the increasing capacity of leading next-generation sequencers, the multiplexing support capabilities of the Sureselect XT platform make it possible for geneticists to interrogate the genome of more samples per study than previously possible.
One of the rate-limiting steps in such studies has been library preparation and target enrichment.
To enable high-throughput sample-processing efficiency, Agilent Automation has released an integrated workstation that automates Sureselect XT library preparation and target enrichment workflows.
This system results in greater throughput - up to 192 samples weekly per workstation, and enables greater laboratory productivity for fast and robust data generation.
The Sureselect XT target enrichment system offers a broad range of complete target enrichment solutions and highly optimised protocols for a diverse set of sequencing methods and platforms.
Sureselect XT products are available for enrichment of target sizes ranging from smaller than 200Kb to more than 50Mb in a single tube.
Protocols now support Illumina end sequencing, paired-end sequencing and indexing protocols in addition to fragment library format, paired-end sequencing and barcoding on the Solid system.